Welcome!
The eDNA Service Center offers processing of environmental DNA samples, assay development, field surveys and training services. The equipment housed in the center will allow users to perform fluorescence-based qPCR analyses, for quantifying DNA concentration and abundances, and plate preparation. In time, the center will also offer nextgen-sequencing eDNA services, including preparation of samples and on-site sequencing of eDNA metabardcodes.
| Hours | Location |
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|
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| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Geneva York |
Manager, eDNA Service Center
|
207-581-2591
|
geneva.york@maine.edu
|
305 Murray Hall
|
| Michael Kinnison |
eDNA Program Lead
|
207-581-2575
|
mkinnison@maine.edu
|
313A Murray Hall
|
| Megan Tardif |
Assistant Director of Finance for CORE
|
207-581-1225
|
megan.tardif@maine.edu
|
153 ESRB-Barrows Hall
|
| Peter Avis, Ph.D. |
CORE Director
|
207-581-2251
|
peter.avis@maine.edu
|
155 ESRB-Barrows Hall
|
| Service list |
| ► eDNA Sample Processing (9) | |||
| Name | Description | Price | |
|---|---|---|---|
| ddPCR |
Absolute quantification of DNA |
Inquire | |
| MinION® |
Long sequencing reads (consumables billed separately). |
Inquire | |
| MiSeq® Sequencing Run |
Next-generation sequencing (Reagent kit billed separately) |
Inquire | |
| PCR |
DNA Amplification |
Inquire | |
| eDNA Sample Filtering |
Per Liter Sample Vacuum filtration of water samples for eDNA collection |
Inquire | |
| eDNA Extraction |
Per Sample Filter Extraction and concentration of eDNA from water sample filters. Specialized kits available per request and charged as add-on. |
Inquire | |
| Sample Clean-Up |
Per Sample Purification of extracted eDNA to increase PCR efficiency. |
Inquire | |
| Targeted eDNA Assay Single Probe Prep + qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using a single Taqman MGB-NFQ probe assay. Rate includes prep work. |
Inquire | |
| Targeted eDNA Assay Double Probe Prep + qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using two Taqman MGB-NFQ probe assays |
Inquire | |
