The eDNA Service Center offers processing of environmental DNA samples, assay development, field surveys and training services. The equipment housed in the center will allow users to perform fluorescence-based qPCR analyses, for quantifying DNA concentration and abundances, and plate preparation. In time, the center will also offer nextgen-sequencing eDNA services, including preparation of samples and on-site sequencing of eDNA metabardcodes.
Hours | Location |
|
edna.core@maine.edu |
Name | Role | Phone | Location | |
---|---|---|---|---|
Geneva York |
Manager, eDNA Service Center
|
207-581-2591
|
geneva.york@maine.edu
|
305 Murray Hall
|
Michael Kinnison |
eDNA Program Lead
|
207-581-2575
|
mkinnison@maine.edu
|
313A Murray Hall
|
Megan Tardif |
Assistant Director of Finance for CORE
|
207-581-1225
|
megan.tardif@maine.edu
|
153 ESRB-Barrows Hall
|
Shane Moeykens |
Interim Co-Director of CORE
|
207-581-3399
|
shane.moeykens@maine.edu
|
Service list |
► eDNA Sample Processing (9) | |||
Name | Description | Price | |
---|---|---|---|
ddPCR |
Absolute quantification of DNA |
Inquire | |
MinION® |
Long sequencing reads (consumables billed separately). |
Inquire | |
MiSeq® Sequencing Run |
Next-generation sequencing (Reagent kit billed separately) |
Inquire | |
PCR |
DNA Amplification |
Inquire | |
eDNA Sample Filtering |
Per Liter Sample Vacuum filtration of water samples for eDNA collection |
Inquire | |
eDNA Extraction |
Per Sample Filter Extraction and concentration of eDNA from water sample filters. Specialized kits available per request and charged as add-on. |
Inquire | |
Sample Clean-Up |
Per Sample Purification of extracted eDNA to increase PCR efficiency. |
Inquire | |
Targeted eDNA Assay Single Probe Prep + qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using a single Taqman MGB-NFQ probe assay. Rate includes prep work. |
Inquire | |
Targeted eDNA Assay Double Probe Prep + qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using two Taqman MGB-NFQ probe assays |
Inquire |