Welcome!
The eDNA Service Center offers processing of environmental DNA samples, assay development, field surveys and training services. The equipment housed in the center will allow users to perform fluorescence-based qPCR analyses, for quantifying DNA concentration and abundances, and plate preparation. In time, the center will also offer nextgen-sequencing eDNA services, including preparation of samples and on-site sequencing of eDNA metabardcodes.
| Hours | Location |
|
|
edna.core@maine.edu |
When shipping samples, please ship samples to:
CORE Environmental DNA Lab
Attn: Alexandra Sacco
University of Maine
5751 Murray Hall Rm 223
Orono, Me 04469
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Alexandra Sacco |
Genetics Facilities Manager
|
alexandra.j.sacco@maine.edu
|
||
| Julie Rutherford |
Fiscal Officer
|
581-1118
|
julie.rutherford@maine.edu
|
|
| Michael Call |
CORE Facilities Manager
|
michael.call@maine.edu
|
||
| Megan Tardif |
CORE Director
|
207-581-1225
|
megan.tardif@maine.edu
|
153 ESRB-Barrows Hall
|
| Service list |
| ► eDNA Sample Processing (9) | |||
| Name | Description | Price | |
|---|---|---|---|
| eDNA Sample Filtering |
Per Liter Sample Vacuum filtration of water samples for eDNA collection |
Inquire | |
| eDNA Extraction |
Per Sample Filter Extraction and concentration of eDNA from water sample filters. Specialized kits available per request and charged as add-on. |
Inquire | |
| Sample Clean-Up |
Per Sample Purification of extracted eDNA to increase PCR efficiency using Zymo PCR Inhibitor Removal Kit. |
Inquire | |
| PCR |
DNA Amplification Charge is per sample |
Inquire | |
| Targeted eDNA Assay Single Probe qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using a single Taqman MGB-NFQ probe assay. |
Inquire | |
| Targeted eDNA Assay Double Probe qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using two Taqman MGB-NFQ probe assays |
Inquire | |
| MiSeq® Sequencing Run |
Next-generation sequencing (Reagent kit billed separately) |
Inquire | |
| MinION® |
Long sequencing reads (consumables billed separately). |
Inquire | |
| ddPCR |
Absolute quantification of DNA |
Inquire | |
| ► Technical Services (1) | |||
| Name | Description | Price | |
| QubitTM Fluorometer Measurement |
DNA quantification |
Inquire | |
