Welcome!
ALERT ATTENTION ⚠️
In observance of the holidays, Agilent CrossLab/iLab Operations Software Support Help Desk will be closed during U.S. hours on Monday, January 20th, 2025. We will resume regular U.S. support hours on Tuesday, January 21st, 2025. For urgent matters, please add "Urgent" to the ticket/email subject or press "1" when prompted to escalate a call on the iLab Support phone, and we will prioritize those requests first.
The eDNA Service Center offers processing of environmental DNA samples, assay development, field surveys and training services. The equipment housed in the center will allow users to perform fluorescence-based qPCR analyses, for quantifying DNA concentration and abundances, and plate preparation. In time, the center will also offer nextgen-sequencing eDNA services, including preparation of samples and on-site sequencing of eDNA metabardcodes.
| Hours | Location |
|
|
edna.core@maine.edu |
When shipping samples, please ship samples to:
CORE Environmental DNA Lab
Attn: Lindsey Stover
University of Maine
5751 Murray Hall Rm 223
Orono, Me 04469
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Lindsey Stover |
Genetics Facilities Technician
|
207-581-2591
|
edna@maine.edu
|
305 Murray Hall
|
| Michael Kinnison |
eDNA Program Lead
|
207-581-2575
|
mkinnison@maine.edu
|
313A Murray Hall
|
| Megan Tardif |
Director of CORE
|
207-581-1225
|
megan.tardif@maine.edu
|
153 ESRB-Barrows Hall
|
| Service list |
| ► eDNA Sample Processing (9) | |||
| Name | Description | Price | |
|---|---|---|---|
| eDNA Sample Filtering |
Per Liter Sample Vacuum filtration of water samples for eDNA collection |
Inquire | |
| eDNA Extraction |
Per Sample Filter Extraction and concentration of eDNA from water sample filters. Specialized kits available per request and charged as add-on. |
Inquire | |
| Sample Clean-Up |
Per Sample Purification of extracted eDNA to increase PCR efficiency using Zymo PCR Inhibitor Removal Kit. |
Inquire | |
| PCR |
DNA Amplification Charge is per sample |
Inquire | |
| Targeted eDNA Assay Single Probe qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using a single Taqman MGB-NFQ probe assay. |
Inquire | |
| Targeted eDNA Assay Double Probe qPCR (4 Replicates) |
Per Sample Batch of 4 qPCR replicates using two Taqman MGB-NFQ probe assays |
Inquire | |
| MiSeq® Sequencing Run |
Next-generation sequencing (Reagent kit billed separately) |
Inquire | |
| MinION® |
Long sequencing reads (consumables billed separately). |
Inquire | |
| ddPCR |
Absolute quantification of DNA |
Inquire | |
| ► Technical Services (1) | |||
| Name | Description | Price | |
| QubitTM Fluorometer Measurement |
DNA quantification |
Inquire | |
