Welcome!
The University of Maine DNA Sequencing facility provides high quality and reliable Sanger sequencing and other services to labs on campus and beyond. Users can choose the degree of in-house processing to accommodate individual needs; providing flexibility for clients of all levels. The facility is in the process of expanding our services so keep an eye out for future developments. We’re happy to answer questions and discuss your research needs.
FOR ALL QUESTIONS, PLEASE CONTACT OUR STAFF AT: dnaseq@maine.edu
Alexandra Sacco, CORE Genetics Facilites Manager
Julie Rutherford, Fiscal Officer of CORE
Michael Call, Facilities Manager of CORE
Megan Tardif, Director of CORE
| Hours | Location |
|
Monday - Friday |
5735 Hitchner Hall |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Alexandra Sacco |
Genetics Facilities Manager
|
alexandra.j.sacco@maine.edu
|
||
| Julie Rutherford |
Fiscal Officer
|
207-581-1118
|
julie.rutherford@maine.edu
|
|
| Michael Call |
Facilities Manager
|
michael.call@maine.edu
|
||
| Megan Tardif |
Director
|
207-581-1225
|
megan.tardif@maine.edu
|
| Service list |
| ► Sanger Sequencing Services (3) | |||
| Name | Description | Price | |
|---|---|---|---|
| Electrophoresis |
Provides capillary electrophoresis of a client prepared plate. |
Inquire | |
| Fragment Analysis |
Electrophoresis and sizing of DNA samples using ABI Genescan Analysis Software. |
Inquire | |
| Sanger Sequencing - Cycle Sequencing |
Cycle sequencing, ETOH/EDTA clean up, and electrophoresis of DNA samples. The lab uses the Big Dye brand of reagents. |
Inquire | |
| ► Sample Preparation (2) | |||
| Name | Description | Price | |
| Normalization |
Calculates and dilutes DNA samples to a concentration suitable for Sanger sequencing or another desired application |
Inquire | |
| Purification- ExoSAP |
Enzymatic purification method provides 100% recovery of amplicons regardless of fragment size. |
Inquire | |
| ► Self-Service Equipment (2) | |||
| Name | Description | Price | |
| Self-Service Equipment Use: Qubit |
Quantification - Qubit Fluorescence based assay is nucleic acid specific and can accurately measure both dilute samples and samples with contaminates. |
Inquire | |
| TapeStation: Agilent 4150 |
The Agilent Tape Station performs automated electrophoresis of DNA or RNA samples and generates |
Inquire | |
