Welcome!
ALERT ATTENTION ⚠️
In observance of a holiday, Agilent CrossLab/iLab Operations Software Support Help Desk will be closed during U.S. hours on Thursday, November 27th and Friday, November 28th, 2025. We will resume regular U.S. support hours on Monday, December 1st, 2025. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/email subject or press "1" when prompted to escalate a call on the iLab Support phone, and we will prioritize those requests first.
The University of Maine DNA Sequencing facility provides high quality and reliable Sanger sequencing and other services to labs on campus and beyond. Users can choose the degree of in-house processing to accommodate individual needs; providing flexibility for clients of all levels. The facility is in the process of expanding our services so keep an eye out for future developments. We’re happy to answer questions and discuss your research needs.
FOR ALL QUESTIONS, PLEASE CONTACT OUR STAFF AT: dnaseq@maine.edu
Alexandra Sacco, CORE Genetics Facilites Manager
Julie Rutherford, Fiscal Officer of CORE
Michael Call, Facilities Manager of CORE
Megan Tardif, Director of CORE
| Hours | Location |
|
Monday - Friday |
5735 Hitchner Hall |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Alexandra Sacco |
Genetics Facilities Manager
|
alexandra.j.sacco@maine.edu
|
||
| Julie Rutherford |
Fiscal Officer
|
207-581-1118
|
julie.rutherford@maine.edu
|
|
| Michael Call |
Facilities Manager
|
michael.call@maine.edu
|
||
| Megan Tardif |
Director
|
207-581-1225
|
megan.tardif@maine.edu
|
| Service list |
| ► Sanger Sequencing Services (3) | |||
| Name | Description | Price | |
|---|---|---|---|
| Electrophoresis |
Provides capillary electrophoresis of a client prepared plate. |
Internal
$1.90
reactions
Academic $2.15 reactions External $2.50 reactions |
|
| Fragment Analysis |
Electrophoresis and sizing of DNA samples using ABI Genescan Analysis Software. |
Internal
$1.90
reactions
Academic $2.15 reactions External $2.50 reactions |
|
| Sanger Sequencing - Cycle Sequencing |
Cycle sequencing, ETOH/EDTA clean up, and electrophoresis of DNA samples. The lab uses the Big Dye brand of reagents. |
Inquire | |
| ► Sample Preparation (4) | |||
| Name | Description | Price | |
| Purification: ExoSAP |
Enzymatic purification method provides 100% recovery of amplicons regardless of fragment size. |
Internal
$4.90
Sample
Academic $5.50 Sample External $6.20 Sample |
|
| Purification: QIAquick |
Spin column purification method provides up to 95% recovery of amplicons between 100bp - 10kb. |
Internal
$5.70
Sample
Academic $6.25 Sample External $7.00 Sample |
|
| Quantification: NanoDrop |
Absorbance-based assay can provide purity ratios such as A260/A280 and identify contaminants. |
Internal
$2.80
Sample
Academic $3.00 Sample External $3.45 Sample |
|
| Quantification: Qubit |
Fluorescence based assay is nucleic acid specific and can accurately measure both dilute samples and samples with contaminates. |
Internal
$4.80
Sample
Academic $5.30 Sample External $6.00 Sample |
|
| ► Technician Time (1) | |||
| Name | Description | Price | |
| GA Lab Technician Hour |
Optional services to include training, consultation, lab safety training, etc. |
Internal
$35.00
/hr
Academic $45.00 /hr External $60.00 /hr |
|
| ► Self-Service Equipment (3) | |||
| Name | Description | Price | |
| Self-Service Equipment Use: Qubit |
Quantification Fluorescence based assay is nucleic acid specific and can accurately measure both dilute samples and samples with contaminates. |
Internal
$1.25
Sample
|
|
| Self-Service: Agilent 4150 TapeStation |
Price is per hour with a 15 min. minimum reservation. The Agilent Tape Station performs automated electrophoresis of DNA or RNA samples and generates |
Internal
$10.00
/hr
|
|
| Self-Service: Agilent Cytation 5 Plate Reader |
Price is per hour with a 15 min. minimum reservation. Agilent Cytation Biotek Multi-Mode Plate Reader. Cytation 5 combines automated microscopy and conventional microplate detection. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast. The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 software provides complete control over all imaging and data capture, plus powerful image and data analysis.
|
Internal
$8.00
/hr
|
|
