Welcome!
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Overview of the CORE Electron Microscopy Lab |
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Overview of the Microscopy and Image Analysis Core |
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Contact Emma Perry, Ph.D. |
Location 11 Murray Hall |
Office Hours Monday - Friday 8am - 4pm Links & Resources: |
Contact Zhengxin Ma, Ph.D. |
Location 369 Hitchner Hall |
Office Hours Monday - Friday 8am - 4pm Links & Resources: |
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The Electron Microscopy Laboratory offers the tools and technical expertise for research and training in microscopy with both light and electron microscopes. We maintain and operate electron microscopes (TEM and SEM with EDS), a confocal laser scanning microscope, and the ancillary equipment needed for the preparation of specimens for EM and other microscopy. Our multiple light microscopes encompass optics for bright-field, phase-contrast, differential-interference, and fluorescence imaging. Technical Services: • Microscope operation—full operation of all electron and light microscopes.
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Established through a COBRE Grant from NIH, the COBRE Microscopy and Image Analysis CORE has two specific aims designed to provide training and access to (1) state-of-the art, high resolution live imaging services and (2) experiment planning, data management and image analysis services. The Microscopy and Image Analysis Core (MIAC) offers cutting-edge imaging capabilities with its Zeiss LSM980 equipped with AiryScan 2. This advanced microscope supports a wide array of applications, including multi-color experiments, live sample imaging, protein colocalization, tiling of large samples, Z-stack 3D/4D imaging, and weak label detection. Additionally, MIAC provides expert guidance in microscopy and image analysis, as well as comprehensive training and support to advance research across various model organisms. For image analysis, the facility is equipped with Imaris software on a high-performance workstation, ensuring robust and sophisticated data analysis. |
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Leadership:
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Robert Wheeler, Ph.D. MIAC Facility Director Robert.wheeler1@maine.edu |
Michael Call CORE Facilities Manager 207-581-3382 Michael.call@maine.edu |
Julie Rutherford CORE Fiscal Officer 207-581-1118 Julie.rutherford@maine.edu |
Megan Tardif CORE Director 207-581-1225 Megan.tardif@maine.edu |
| Name | Role | Phone | Location | |
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| Emma Perry |
Microscopy Laboratory Manager
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207-581-2566
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emma.perry@maine.edu
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11 Murray Hall
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| Zhengxin Ma |
Microscopy & Image Analysis Core Facility Manager
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207-581-2802
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zhengxin.ma@maine.edu
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282 Hitchner Hall
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| Service list |
| ► CORE EML Equipment (6) | |||
| Name | Description | Price | |
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| Raith |
Internal
$20.00
/hr
External $30.00 /hr Academic $25.00 /hr |
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| AMR 1820 SEM |
Resolution: 5 nm, Billed per hour |
Inquire | |
| iXRF EDS |
iXRF EDS specimen elemental analysis |
Inquire | |
| Zeiss NVision 40 SEM Usage |
Resolution: 1.2 nm, Billed per hour |
Inquire | |
| EDAX EDS |
EDAX EDS specimen elemental analysis |
Inquire | |
| Tecnai G2 BioTWIN TEM |
Electron beam instrument for high resolution imaging of sectioned material and nano-scale particulates |
Inquire | |
| ► CORE EML Sample Prep Equipment (6) | |||
| Name | Description | Price | |
| Autostainer |
Automated stainer for use in histology slide prep. |
Internal
$20.00
Sample
External $30.00 Sample Academic $25.00 Sample |
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| Critical Point Dryer |
Biological specimen drying system for imaging Billed per run |
Inquire | |
| Denton DV502 Vacuum Evaporator |
Carbon & Metal coating for SEM and TEM specimens Billed per run |
Inquire | |
| Glow Discharge |
Grid preparation Billed per run |
Inquire | |
| Sputter Coater |
Metal coating for SEM Per run |
Academic
$30.00
Run
External $35.00 Run |
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| Ultramicrotome |
Specimen sectioning for light and electron microscopy Billed per hour |
Inquire | |
| ► Microscopy and Image Analysis CORE (4) | |||
| Name | Description | Price | |
| Airyscan2 Zeiss LSM 980 Confocal |
Zeiss LSM 980 – Airyscan 2: The inverted laser scanning confocal microscope is ideal for diverse range of applications for confocal and super resolution imaging including multi-color experiments, live sample imaging, protein colocalization, tiling of large samples, Z-stack 3D/4D imaging, and weak label detection. The microscope is equipped with environmental control (temperature, humidity and CO2) and can image live BSL-2 samples. The motorized XY stage can hold samples in slides, 35 mm dishes, chambered slides, and well-plates. |
Inquire | |
| Image Analysis Workstation |
Our Imaris workstation, built by Puget Systems, is fully equipped with all Imaris packages including ClearView deconvolution and Imaris Stitcher. It features a powerful AMD Ryzen Threadripper PRO 5975WX 32-core processor, 512 GB of RAM, and NVIDIA RTX 4000 Ada Generation graphics card, ensuring top-notch performance for advanced imaging data analysis. |
Inquire | |
| Imaris Image Analysis Software & Image Analysis Workstation |
Imaris is a user-friendly and extremely powerful package that excels in visually presenting 3D and 4D datasets. It boasts robust capabilities for conducting quantitative analyses on multi-dimensional images, and seamlessly manages a wide array of commercial fluorescence microscopy file formats. Our Imaris workstation, built by Puget Systems, is fully equipped with all Imaris packages including ClearView deconvolution and Imaris Stitcher. It features a powerful AMD Ryzen Threadripper PRO 5975WX 32-core processor, 512 GB of RAM, and NVIDIA RTX 4000 Ada Generation graphics card, ensuring top-notch performance for advanced imaging data analysis. |
Inquire | |
| Training/Assistance: Ma |
Training/Assistance by Dr. Ma: General consultation – biological sample preparation, planning and strategy for robust experimental organization, optimization of image acquisition, planning for publication-ready data analysis, preparation of materials & methods for peer-reviewed publications Zeiss LSM980 Assistant use/training – equipment usage with hands-on assistance for image acquisition Image acquisition by MIAC staff – hourly rate to obtain images/data for a user Image analysis (Imaris and ImageJ) Assistant use/training – software usage with hands-on assistance Image analysis by MIAC staff – hourly rate to conduct imaging analysis for a user |
Inquire | |
